14.11 Red cell folate

Red cell folate data available for STOP cohort only.

14.11.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	85.988271	1	<0.0001	***
ns(rcfolate, 3)	4.943268	3	0.2	ns
GA_samp	87.352352	1	<0.0001	***

hPL (μg/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	3.511184	1	0.06	ns
ns(rcfolate, 3)	1.672576	3	0.6	ns
GA_samp	1649.063858	1	<0.0001	***

GH2 (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	141.614158	1	<0.0001	***
ns(rcfolate, 3)	5.404287	3	0.1	ns
GA_samp	765.463640	1	<0.0001	***

Insulin (uU/ml)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate	1	Inf	0.303	0.303	0.6	ns
GA_samp	1	Inf	1.955	1.955	0.2	ns

HOMA-IR

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate	1	Inf	0.387	0.387	0.5	ns
GA_samp	1	Inf	1.423	1.423	0.2	ns

Glucose (mmol/L)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate	1	Inf	0.356	0.356	0.6	ns
GA_samp	1	Inf	1.484	1.484	0.2	ns

14.11.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)

Var	Effect	P
(Intercept)	21.49 (11.25, 41.07)	<0.0001
ns(rcfolate, 3)1	0.89 (0.60, 1.33)	0.57
ns(rcfolate, 3)2	0.79 (0.24, 2.63)	0.70
ns(rcfolate, 3)3	1.07 (0.93, 1.25)	0.34
GA_samp	1.13 (1.10, 1.16)	<0.0001

hPL (μg/ml)

Var	Effect	P
(Intercept)	1.44 (0.98, 2.10)	0.06
ns(rcfolate, 3)1	1.10 (0.87, 1.40)	0.43
ns(rcfolate, 3)2	1.40 (0.69, 2.85)	0.36
ns(rcfolate, 3)3	0.99 (0.91, 1.08)	0.87
GA_samp	1.36 (1.34, 1.38)	<0.0001

GH2 (ng/ml)

Var	Effect	P
(Intercept)	0.05 (0.03, 0.08)	<0.0001
ns(rcfolate, 3)1	1.05 (0.77, 1.45)	0.75
ns(rcfolate, 3)2	1.59 (0.61, 4.14)	0.34
ns(rcfolate, 3)3	0.93 (0.83, 1.04)	0.21
GA_samp	1.33 (1.30, 1.36)	<0.0001

Insulin (uU/ml)

Var	Effect	P
(Intercept)	9.80 (5.14, 18.67)	<0.0001
ns(rcfolate, 3)1	0.89 (0.61, 1.30)	0.56
ns(rcfolate, 3)2	0.82 (0.26, 2.59)	0.74
ns(rcfolate, 3)3	1.17 (0.99, 1.38)	0.06
GA_samp	1.02 (0.99, 1.06)	0.16

HOMA-IR

Var	Effect	P
(Intercept)	2.21 (1.09, 4.48)	0.03
ns(rcfolate, 3)1	0.87 (0.58, 1.32)	0.51
ns(rcfolate, 3)2	0.76 (0.21, 2.72)	0.67
ns(rcfolate, 3)3	1.17 (0.99, 1.39)	0.07
GA_samp	1.02 (0.99, 1.06)	0.23

Glucose (mmol/L)

Var	Effect	P
(Intercept)	5.08 (4.36, 5.93)	<0.0001
ns(rcfolate, 3)1	0.99 (0.90, 1.09)	0.82
ns(rcfolate, 3)2	0.94 (0.68, 1.29)	0.69
ns(rcfolate, 3)3	1.03 (1.00, 1.07)	0.07
GA_samp	1.00 (0.99, 1.00)	0.22

14.11.3 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.11.4 Plots